Studies on the regeneration of protochlorophyllide after brief illumination of etiolated bean leaves.
نویسندگان
چکیده
The effects of various inhibitors of nucleic acid and protein synthesis on protochlorophyllide synthesis in dark-grown Phaseolus vulgaris var. Red Kidney have been studied. Actinomycin D, chloramphenicol, and puromycin inhibit the regeneration of protochlorophyllide holochrome (detected as a 650 mmu absorption peak) in vivo in darkness after photoconversion of endogenous protochlorophyllide a to chlorophyllide a; this inhibition does not occur in similarly treated leaves supplied with delta-aminolevulinic acid.These data suggest that the regeneration of protochlorophyllide results from the synthesis of RNA and enzymes required for the production of delta-aminolevulinate.
منابع مشابه
Changing ratios of phototransformable protochlorophyll and protochlorophyllide of bean seedlings developing in the dark.
Protochlorophyll (Pchl) and protochlorophyllide (Pchlide) are at comparable levels in 2-day-old (young) etiolated bean leaves (Phaseolus vulgaris L. var. Red Kidney). During subsequent development in the dark, both pigments increase, but the rate of Pchlide increase is greater than that of Pchl, leading to the commonly observed predominance of Pchlide beyond 7 days (old leaves). Both protopigme...
متن کاملChlorophyll Formation in Greening Bean Leaves during the Early Stages.
In the evolution of the absorption spectrum of etiolated bean leaves (Phaseolus vulgaris L.) following illumination, a rapid photoconversion of 50% or more of the active protochlorophyllide at room temperature is followed by a shift of the chlorophyll(ide) absorption maximum: C(678)--> -->C(684)-->C(672 nm). Kinetic studies at 2 C and the absence of an isosbestic point provide evidence for an i...
متن کاملImmunological distinction between fraction I protein and protochlorophyllide holochrome.
Ribulose 1,5-diphosphate carboxylase (RuDP carboxylase) has been shown to be the major component of the Fraction I protein of leaves (5, 6). Protochlorophyllide associated with a protein, i.e. protochlorophyllide holochrome (PCH), has been isolated from etiolated bean leaves (1, 9,10); upon illumination in zirvo or in vitro it is transformed into chlorophyllide holochrome. Several investigators...
متن کاملThe Conversion of Photoinactive Protochlorophyllide(633) to Phototransformable Protochlorophyllide(650) in Etiolated Bean Leaves Treated with delta-Aminolevulinic Acid.
The relationship of phototransformable protochlorophyllide to photoinactive protochlorophyllide has been studied in primary leaves of 7- to 9-day-old dark-grown bean (Phaseolus vulgaris L. var. Red Kidney) seedlings. Various levels of photoinactive protochlorophyllide, absorbing at 633 nm in vivo, were induced by administering delta-aminolevulinic acid to the leaves in darkness. Phototransforma...
متن کاملFine Structural Changes in Proplastids during Photodestruction of Pigments
Etiolated bean leaves supplied delta-amino-levulinic acid in the dark synthesize large amounts of protochlorophyllide which is not converted to chlorophyllide upon illumination of the leaves. The fine structure of the proplastids is not affected by the treatment. When leaves containing "inactive" protochlorophyllide are exposed to light of 700 ft-c for 3 hours, they lose practically all their g...
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عنوان ژورنال:
- Plant physiology
دوره 42 6 شماره
صفحات -
تاریخ انتشار 1967